Taking well-X, a typical core well of the Wufeng-Longmaxi Formation in Southern Sichuan Basin, as an example, based on core observation, geochemical analysis, X-ray diffraction, physical properties testing, argon ion polishing SEM, hydrocarbon generation simulation and other testing methods, and by using the research idea of “source-storage coupling”, the properties of the core segment as the “source” and the “storage” respectively are analyzed, and the mechanism of “source-storage coupling” is further discussed. The research results show that the bottom shale in Wufeng-Longmaxi Formation is characterized by sapmpelic kerogen, abundant organic matter, and high degree of thermal evolution, in which dry gas mainly generate. The average porosity of matrix is 6.5 %, mainly are inorganic pores. The inorganic pore types are mainly intergranular pore, clay mineral pore, feldspar alteration pore and pyrite intergranular pore. The average amount of adsorbed gas under formation temperature and pressure conditions is 0.9 m3/t. The average permeability is 0.38×10-3 μm2, which means it belongs to an ultra-low permeability reservoir. Generally speaking, the well-X has sufficient “source” at the bottom of the Wufeng-Longmaxi Formation with large amount of generated gas. The current stagnant gas volume is only about 54.8 % of the amount of generated gas, and the matrix pores are mainly inorganic pores. Meanwhile, organic pores are an important supplement. Free gas is the main body. The development of organic-rich shale section improves the permeability of the reservoir. At the same time, the biogenic siliceous minerals enhance the brittleness of the reservoir and make the reservoir easy to be fractured. Therefore, the organic-rich shale section is the best source-storage coupling interval, and also is the main interval of shale gas exploration and development and the key target layer of a horizontal well.